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Alliance iS HPLC Systems User Guide
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Alliance iS HPLC Systems User Guide
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PDA detector features

2D and 3D acquisition

2D and 3D acquisition
Topic LCI-USG-0218, Last Updated 11/07/2024

The Alliance iS system PDA detector is capable of both 2D and 3D acquisition.

The PDA detector can simultaneously collect two types of data channels: spectra (3D) and chromatograms (2D). For best results in library matching and peak-purity analysis, set the 3D resolution to 1 nm.

For chromatograms (2D data), select a resolution to optimize signal amplitude, baseline noise, and linear dynamic range. When the monitoring wavelength for an analyte corresponds to a peak’s lambda max, increasing bandwidth tends to decrease peak height while reducing baseline noise and linear dynamic range.

Tip: A resolution of 4 nm is effective for many analytes.
Figure 1: Resolution comparison for caffeine

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