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Alliance iS HPLC Systems User Guide
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Alliance iS HPLC Systems User Guide
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TUV detector features

Cuvette operations

Cuvette operations
Topic LCI-USG-0008, Last Updated 11/08/2024

The detector's cuvette option is used to measure the absorbance spectrum of a sample in a cuvette.

Note: This section pertains only to use of the optional cuvette.

To generate and store a spectrum:

  1. Acquire a Blank scan, which measures the absorbance of the contents of the cuvette over the desired wavelength range.
  2. Acquire a sample (Absorbance) scan, which measures the absorbance of the analyte dissolved in solution.
    Result: The detector subtracts the Blank scan from the sample scan to create a sample spectrum.

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