Calibrating the PDA detector

Calibrate the detector if wavelength verification fails with a properly flushed flow cell.

To perform detector calibration, prime the system and run flow for 10 minutes to flush the flow cell with solvent and ensure that it is fully wetted. A 90:10 water/acetonitrile mixture is recommended at 0.5 mL/min. The lamp should be warmed up for a minimum of 5 minutes and in the "ON" state prior to calibration.

If buffers have been used recently in the flow cell, it is recommended that you first flush with 10 mL of HPLC-grade water, followed by 10 mL of a low-surface-tension solvent such as methanol (provided it is miscible with the previous mobile phase).

Contents

• Erbium calibration
  
• Mercury calibration