Replacing the primary check valve

Replacing the primary check valve
Topic LCI-USG-0227, Last Updated 02/26/2025

Users or Waters field service personnel can replace the pump primary check valve.

Warning: Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials. Consult the Safety Data Sheets regarding the solvents you use. Additionally, consult the safety representative for your organization regarding its protocols for handling such materials.
Note: To avoid contaminating system components, wear clean, chemical-resistant, powder-free gloves when performing this procedure.

Required tools and materials

  • Chemical-resistant, powder-free gloves
  • 1/2-inch open-end wrench
  • Replacement primary check valve assembly

To replace the primary check valve:

  1. Flush the pump with nonhazardous solvent that is miscible with the present mobile phase.
  2. Power-off the system. See Powering-off the system.
  3. Open the pump compartment door.
  4. Loosen the PEEK inlet filter nut from the PEEK inlet filter fitting. Do not remove the nut from the fitting.
    Figure 1: Loosening the PEEK inlet filter nut from the PEEK inlet filter fitting
    PEEK inlet filter fitting
    PEEK inlet filter nut
  5. Unscrew and remove the PEEK inlet filter fitting and PEEK inlet filter nut from the primary check valve.
  6. Note: When you remove the check valve assembly, ensure that the PEEK washer, which is normally on the top face of the check valve, does not remain in the pump head.
    Use the 1/2-inch open-end wrench to loosen the check valve, and then remove the check-valve assembly from the pump head.
    Figure 2: Removing the primary check valve from the pump head
    Place 1/2-inch open-end wrench here
  7. Unpack the new check valve.
    Note: Verify that there is a PEEK gasket in the groove on top of the check valve and that the chamfered side is pointing up. The check valve can't be installed without the gasket.
  8. Insert the check valve assembly into the pump head, tighten the check valve nut with your fingers to the extent possible, and then use the 1/2-inch wrench to tighten the nut an additional 1/8-turn.
  9. Finger tighten the PEEK inlet filter fitting into the primary check valve.
    Note: The following image shows the pressure transducer removed for clarity. The pressure transducer is not removed as part of this procedure.
    Figure 3: Reattaching the PEEK inlet filter fitting and the PEEK inlet filter nut
    Primary check valve
    PEEK inlet filter fitting
    PEEK inlet filter nut
  10. Finger-tighten the PEEK inlet filter nut to the extent possible.
  11. Close the pump compartment door.
  12. Power-on the system.
  13. Prime the pump.

Need Help? Connect with a Waters expert. 


To avoid risk of personal injury and to prevent damage to laboratory equipment, always operate your Waters products in accordance with the applicable operational and safety information, your organization’s standard operating procedures, and local regulations.

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