Buffered solvents

Topic LCI-USG-0038, 最近更新 11/28/2023

When using a buffer, choose good quality reagents, filtering them through a 0.2-μm membrane filter.

Recommendation: To discourage microbial growth, replace 100% mobile aqueous phase daily.

Adjust the pH of aqueous buffers. Filter them to remove insoluble material, and then blend them with appropriate organic modifiers. After you use a buffer, flush it from the pump by wet priming using at least five system volumes of HPLC-grade distilled or deionized water.

For shutdowns of more than a day's duration, flush the pump with a 20% methanol (MeOH)/water solution to prevent microbial growth.

See also: For information on preventing contamination, refer to Controlling Contamination in LC/MS Systems (715001307) on the Waters website ( www.waters.com).

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Buffered solvents