Transferring methods

The Alliance iS HPLC System can produce results comparable to those achieved on many other HPLC systems. However, when moving a regulated method from one system to another, typically no modifications to the instrument method can be made. For example, the column type (diameter) specified for a transferred method cannot be changed. Even with the same column type, results may not always be identical. If there is a significant difference in retention time, the dwell volume can be compensated by adjusting the gradient start relative to the injection. Extra-column effects can also be modified.

See the Dwell Volume and Extra-Column Volume: What Are They and How Do They Impact Method Transfer white paper (720005723EN) on www.waters.com.