Measuring dwell volume

When transferring a gradient LC method, you can produce comparable retention times by measuring dwell volume on both systems. The dwell volume is the system volume between the point where the gradient is formed and the column inlet.

You can measure dwell volume using the midpoint of a 0–100% gradient. To do so, run a gradient between two identical solvents, A and B, spiking the B solvent with a marker. Perform the measurement after configuring the system without the column for the instrument method being transferred, replacing the column with a low-volume restrictor to ensure proper pump function.

See also: “Measuring system volume for methods transfer” in the Empower online Information System.